Proliferative effect of aqueous extract of Hyptis fructicosa in l | 1826
Biochemistry & Pharmacology: Open Access

Biochemistry & Pharmacology: Open Access
Open Access

ISSN: 2167-0501

Proliferative effect of aqueous extract of Hyptis fructicosa in liver regeneration in rats after partial hepatectomy

International Conference on Pharmacognosy, Phytochemistry & Natural Products

October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Aloisio Ferreira Pinto Neto

Accepted Abstracts: Biochem & Pharmacol

Abstract :

Objective: To evaluate the effects of aqueous extract of Hyptis fructicosa in liver regeneration after partial hepatectomy in rats. Materials and methods : The study was conducted in 16 Wistar rats (300 to 450 g). All animals were randomly divided into two groups, which consisted of eight animals each: control group (CG), the rats received water for 4 days and the HF group, where rats received aqueous extract of Hyptis fructicosa for 4 days at a dose of 100 mg / kg / day. In this consecutive day treatment, the animals of both groups were submitted to partial hepatectomy approximately 67% of the liver. All operations were performed under inhalation anesthesia with ether. After 24 hours, they underwent a new operation to remove the liver remanascente. Then they were sacrificed. Liver regeneration was evaluated by PCNA immunohistochemistry using monoclonal anti-PCNA primer (PC-10; DAKO A / S, Glostrup, Denmark). All data are expressed as mean � standard deviation. Statistical comparisons between groups were performed by Mann-Whitney test (p<0.05). Results : The rate of liver regeneration, which was assessed by proliferating cell nuclear antigen (PCNA), using monoclonal anti-PCNA primary, was significantly higher on the HF group than on the control group (CG). On the HF group this rate was 53.56%�18.91 while the CG presented index 21.12�8.29%. With the use of aqueous extract of Hyptis fructicosa , liver regeneration increased by about 2.5 times. Conclusion : The present study shows that the aqueous extract of Hyptis fructicosa dose of 100 mg/kg/day stimulated liver regeneration in rats.

Biography :

Aloisio Ferreira Pinto Neto has completed his graduation at the age of 26 years from the Universidade Federal de Sergipe, one of the ten best medical universities of the country.