Drug Designing: Open Access
Open Access
ISSN: 2169-0138
+44 1223 790975
ISSN: 2169-0138
+44 1223 790975
Mehmet Ozler, Nail Ersoz , Turgut Topal, Bulent Uysal, Sukru Oter and Ahmet Korkmaz
Scientific Tracks Abstracts: Drug Des
Objectives: Melatonin, the primary substance secreted by the pineal gland, is a molecule common to all aerobic life forms from
unicellular organisms to mammals. It has long been recognized that melatonin possesses the ability to increase gene expression and
the activities of various antioxidant enzymes. However, the activities of these enzymes do not depend exclusively on melatonin. Using
a peritoneal adhesion model in rats, we measured several oxidant-antioxidant parameters in postmortem brain tissue. We believe
these parameters are indicative of the impact of peritoneal trauma on the brain.
Methods: During this study, 21 Sprague-Dawley male rats were divided into three groups and a pinealectomy procedure was
performed on two of the groups. Fifteen days post-surgery, the peritoneal adhesion model was established by making an incision in
the cecum of the rats, followed by suturing. One of the groups that received pinealectomies was administered a single dose (5 mg/kg)
of oral melatonin for 15 days. Treatment began immediately following the operation and continued for 15 days. At the end of the 15
days, the rats’ abdomens were opened under anesthesia. Apart from evaluation of adhesion lesions, their craniums were opened and
their brain tissues were excised. After 15 days, and while under anesthesia, surgery was performed to examine the peritoneal adhesion
site and to remove the brain. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels
were measured in these tissues.
Results: Whereas brain MDA levels significantly increased in the pinealectimized rats, a significant reduction in MDA was observed in
the group that received melatonin treatment. SOD and GSH-Px enzyme activities significantly decreased following the pinealectomy
procedure. When these groups were given melatonin, GSH-Px enzyme activities nearly increased to control levels; however, SOD
enzyme activities remained significantly lower than the control group.
Conclusion: This study clearly demonstrates that the pinealectomy procedure initiates an oxidative stress response in the rat brain,
and that administration of melatonin can mitigate this effect.
He was graduated from 9 Eylul University Medical School at 1997. He has got his Ph.D. degree at 2005 from the department of physiology at Gulhane School of Medicine.
He was promoted to Associated Professor at 20012. He worked at University of North Carolina at Chapal Hill a year. His research interests are melatonin, medical ozone
and hyperbaric oxygen therapy. He looked for the healing mechanism of oxygen based bio-oxidative therapeutics (i.e., medical ozone and hyperbaric oxygen therapies)
in a variety of experimental inflammatory and oxidative stress models. His laboratory is also focused on anti-oxidative and anti-nitrosative efficacy of melatonin in several
experimental models.