Journal of Pharmaceutical Care & Health Systems
Open Access
ISSN: 2376-0419
ISSN: 2376-0419
Sherif R Abdel-Aal1, Amel M Kamal2, Zeinab T Abdel-Shakour1, Amany Saleem3 and Eman G Haggag2
Posters-Accepted Abstracts: J Pharma Care Health Sys
Ipomoea carnea was recorded along canals and drains, road sides, railways, waste lands and fluid edges in the Nile Delta. GC/MS was used for qualitative and quantitative analyses of lipoidal content of the leaves and flowers of I. carnea. 32 components representing 83.548% were identified in the fatty acid methyl esters of the leaves of I. carnea while 23 components representing 84.424% were identified in the fatty acid methyl esters of the flowers. On the other hand, 22 components were identified in the unsaponifiable matter of the leaves representing 83.398%, while 25 components were identified in the unsaponifiable matter of the flowers representing 91.069 %. HPLC was used for assay of rutin in leaves and flowers methanol extract of I. carnea. The leaves methanol extract showed higher concentration of rutin (8.85 mg/g dry wt.) than flower methanol extract (2.72 mg/g dry wt.). The total phenolic compounds, the in vitro and in vivo antioxidant properties and the antimicrobial activity against gram-positive and gram-negative bacteria, fungi and their minimum inhibitory concentration (MIC) were evaluated. The results showed that the methanol extract of flower contained higher phenolic compounds than ethanol extract of leaves (9.331and 6.6348 mg (GAE)/g dry wt., respectively). While the methanol extract of leaves possessed higher DPPH radical scavenging activity than methanol extract of flowers (IC50=1.608 and 4.861 mg/ ml respectively). The flowers extract showed higher antibacterial activity against Escherichia coli, Streptococcus pneumonia and Bacillis subtilis than that of leaves. On the other hand, the leaves exhibited higher antifungal activity than that of the flowers against Aspergillus fumigates. MIC values of the flowers and leaves extracts were ranged 0.98�??15.63 and 7.81 to 125 μg/ml, respectively. The extracts were tested for cytotoxic activity against liver, breast and colon carcinoma cell lines; leaves extract showed potent cytotoxic activity against breast carcinoma cell lines (IC50=7.4 μg/ml while it showed mild cytotoxic effect on liver and colon carcinoma cell lines (IC50=23 and 35 μg/ml) comparing to doxorubicin while the flower extract showed cytotoxic activity against breast carcinoma cell lines (IC50=44.2 μg/ml) and no activity against liver and colon carcinoma cell lines.