Current Synthetic and Systems Biology

Current Synthetic and Systems Biology
Open Access

ISSN: 2332-0737

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Olive biotechnology: The assessment of tissue culture induced genetic variability in olive (Oleaeuropaea) by molecular markers


International Conference on Synthetic Biology

September 28-29, 2015 Houston, USA

Masoud Sheidai and BanafshehKangarlo

ShahidBehesti University, Iran

Scientific Tracks Abstracts: Curr Synthetic Sys Biol

Abstract :

Olive is one of the most important tree crops of the country and it is cultivated in many places. Propagation of true to type olive plants is important for many olive companies and farmers in the country and for this reason genetic analysis ofrandomly selected tissue culture regenerated olive plants of cultivars Kroneiki, Zard, Roughani and X was performed and compared with the mother plant. We used Cp-DNA and RAPD molecular markers to check genetic fidelity versus somaclonal variation. Both RAPD and Cp-DNA analyses revealed that some of the regenerated plants differed extensively in their genetic content. These plants were placed in different clusters far from the mother plants in NJ tree and TNT tree obtained from these two molecular markers. We identified also some regenerated plants that were true to types of the mother plants. These plants also differed in their morphological features. Genetic and morphological changes were more extensive with increase in subculture numbers. Different uses of these two types of plants will be discussed.

Biography :

Masoud Sheidai has completed his PhD at the age of 28 years from Poona University India and Sabbatical studies from UBC University in Canada. He is now proffessor of Biology in ShahidBeheshti University, Tehran, Iran. He has published more than 125 papers in reputed journals and has been serving as an editorial board member of repute. Gene Conserve, and Bidiversity journals.

Email: msheidai@yahoo.com

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