Current Synthetic and Systems Biology
Open Access
ISSN: 2332-0737
+44-20-4587-4809
ISSN: 2332-0737
+44-20-4587-4809
Vitaly K Koltover
Russian Academy of Sciences, Russia
Posters-Accepted Abstracts: Curr Synthetic Sys Biol
Cell structures are composed from atoms of chemical elements, many of which have magnetic and nonmagnetic stable isotopes. The question arises if living cells can perceive the difference between magnetic and non-magnetic isotopes of chemical elements. Of special interest is cation of magnesium as cofactor of ATP synthesis and hydrolysis, etc. Among three stable isotopes of magnesium, 24mg, 25mg and 26mg with natural abundance about 79, 10 and 11 %, only 25mg has the nuclear spin (I = 5/2) and, hence, creates the nuclear magnetic field, whereas 24mg and 26mg have no nuclear spin (I = 0). The nuclear spin catalysis was revealed in experiments with S. cerevisiae. After UV irradiation, the rate constant of post-radiation recovery has turned out to be twice higher for the cells enriched with 25mg than for the cells enriched with the nonmagnetic isotope. Then, in collaboration with Ukrainian colleagues from Palladin Institute of Biochemistry, we studied effects of the magnesium isotopes on ATP hydrolysis driven by myosin, one of the most important molecular motors, isolated from myometrium. It was revealed that the enzyme activity is 2��?2.5 times higher in the reaction media enriched with 25mg as compared to the activity in the media enriched with the spinless 24mg or 26mg. These findings demonstrate the possibility to exert novel, based on the stable magnetic isotopes, ways of control over efficiency and reliability of biocatalysts for medicine and engineering.
Email: koltover@icp.ac.ru