Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
ISSN: 2155-9880
Dong Hoon Kang, H Jo, D J Lee, S W Kang and C H Ha
University of Ulsan, South Korea
Georgia Institute of Technology and Emory University, USA
Ewha Woman′s University, South Korea
Posters & Accepted Abstracts: J Clin Exp Cardiolog
Atherosclerosis is a chronic, inflammatory disease and the main cause in cardiovascular disease (CVD). Atherosclerotic lesions form at specific regions of the arterial tree. Disturbed blood flow plays a major role in the regional localization of atherosclerosis and oxidative stress is considered a risk factor of endothelial cell (EC) in atherosclerotic region. However, the H2O2-mediated mechanosensory mechanism by blood flow is poorly understood. Here we investigated the role of redox complex in ECs as new mechanosensor of blood flow. NOX4 was found to be the most abundant isoform in cultured HAECs and NOX4-derived H2O2 inhibited VEGFR2 activity when peroxiredoxin II (PrxII) was absent. Unexpectedly, the level of NOX4 expression in cultured HAECs was dramatically decreased by laminar shear stress. Unlike cultured mouse aortic endothelial cells (MAEC), the NOX4 level was similar to NOX2 level in freshly-isolated VECs from mouse aorta and the disturbed flow by partial ligation in mouse aorta up-regulated NOX4 expression. Therefore, our study suggests that disturbed blood flow may result in redox imbalance by up-regulating NOX4 expression in VECs, which in turn inhibits VEGFR2 activation.
Dong Hoon Kang has completed his graduation from Seoul National University, PhD from Kyunghee University and Postdoctoral Studies from Ewha Womans University, South Korea. He is currently a Research Associate of Asan Medical Center and has published more than 20 papers about RTK signaling in cardiovascular system.
Email:ref423@gmail.com