Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 1478 350008

Nanoparticle toxicity assessment: A lesson learnt from fly

7th Euro-Global Summit on Toxicology & Applied Pharmacology

October 24-26, 2016 Rome, Italy

Monalisa Mishra, Basanti Ekka, S Aurosman Pappus, Unnikannan P, Abhinandan Patnaik, Priyabrat Dash and Debabrat Sabat

National Institute of Technology, Rourkela, India
IISER, Kolkata, India
IISER, Thiruvananthapuram, India

Posters & Accepted Abstracts: J Clin Toxicol

Abstract :

In recent era, wide usage of nanoparticles (NPs) in domestic items, medicines, cosmetics, body implants, etc., has increased the threat to the live cell as well as the genetic material. To understand the mechanistic role of nanoparticle toxicity in development and behavior, Drosophila appeared to be a superior model organism. We tested the toxicity of three chemically synthesized nanoparticles: Titanium, hydroxyapatite and zirconia using Drosophila. Since all the chosen nanoparticles are administered orally, we have chosen oral route of administration for this study. The NPs, after oral route of exposure accumulates in the gut, crosses the barrier of periotrophic membrane by inducing apoptosis as confirmed via SEM, trypan blue, 2��?,7��?-dichlorofluorescein and DAPI staining. The toxicity of theses NPs resulted developmental delay, with decrease in pupa count and fly hatching along with weight loss. The adult fly hatched from the treatment vial shows increasing phenotypic defect like loss of thorax bristle (macrochaetes), incomplete wing venation, affected eye (ommatidia). Besides phenotypic defect an altered behavioral phenotypes like larva crawling or adult climbing were also observed. Both phenotypic as well as behavioral assay clearly represented the affected development of neurons, which could be due to affected signaling pathway like Notch, Wnt, EGFR, etc. The current study proved that NPs can alter the development of sensory organs in Drosophila. The nanoparticles toxicity should be accounted before its wide use in food and medicine.

Biography :