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Multifunctional N-cinnamoyl-memantine analogues as potential anti | 60733
Organic Chemistry: Current Research

Organic Chemistry: Current Research
Open Access

ISSN: 2161-0401

+44 1478 350008

Multifunctional N-cinnamoyl-memantine analogues as potential anti-alzheimer's disease agents


18th European Organic Chemistry Congress

July 18, 2022 | Webinar

Maya Chochkova

South-West University, Bulgaria

Posters & Accepted Abstracts: Organic Chem Curr Res

Abstract :

overcome the oxidative stress might reduce the risk of development of this severe pathology. Objective: In this study we aim to link together memantine (Mem) with 3,4-dihydroxy, alfa-methyl, 3-methyl, 3-NO2, 4-NO2, 4-Cl-3-NO2 substituted cinnamic acids (CA) by applying TBTU reagent. The newly compounds have been screened in vitro for their neuroprotective effects. Methods: Measurment of neuroprotective effects of amides against copper-induced toxicity in APPswe cells APPswe cells were seeded into 96-well plates at a density of 5×104 cells/well. APPswe cells were divided into three groups: control group (DMEM/F12 medium), copper-injured group (300 μM copper; model group), and copper-injured groups treated with compounds, in which cells were treated with 0.032 μΜ, 0.16 μΜ, 0.8 μΜ, 4 μΜ, 20 μΜ, and 100 μΜ tested compounds. The cell viability of APPswe cells was measured at 36 h incubation by MTS assay. Measurment of neuroprotective effects against glutamate-induced toxicity in SH-SY5Y cells The SH-SY5Y cells were seeded into 96-well plates at a density of 5×104 cells/well. Cells were divided into three groups: control group (DMEM medium), glutamate-injured group (7 mM glutamate; model group), and glutamate-injured groups treated with compounds, in which model groups were treated with 0.032 μΜ, 0.16 μΜ, 0.8 μΜ, 4 μΜ, 20 μΜ, and 100 μΜ tested compounds. The cell viability of SH-SY5Y cells was measured at 48 h incubation by MTS assay. Results: The results of copper-induced toxicity reveal that amongst the tested amides, compounds CA(3- NO2)-Mem, CA(4-Cl-3-NO2)-Mem, CA(4-NO2)-Mem, CA(alfa-CH3)-Mem displayed neuroprotective effects, ranging from 0.032 μΜ to 20 μΜ, whereas CafA-Mem and CA(3-CH3)-Mem did not show neuroprotective effects on APPswe cells. Moreover, amides CA(3-NO2)-Mem, CA(4-Cl-3-NO2)-Mem, and CA(alfa-CH3)-Mem treated at 0.032 μΜ increased cell viability against glutamate-induced neurotoxicity in SH-SY5Y cells. Conclusion: Combined, these active compounds may have neuroprotective effects with a correlation with the glutamate receptors.
References :
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Biography :

Maya G. Chochkova is affiliated to South-West University. she is a recipient of many awards and grants for her valuable contributions and discoveries in major area of subject research. Her international experience includes various programs, contributions and participation in different countries for diverse fields of study. Her research interests include Antioxidant Activity, Antioxidant Assays, Alkaloids, Antioxidants and DPPH.

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