Drug Designing: Open Access
Open Access
ISSN: 2169-0138
+44 1223 790975
ISSN: 2169-0138
+44 1223 790975
Charlette Tiloke, Alisa Phulukdaree, Robert M Gengan and Anil A Chuturgoon
University of KwaZulu-Natal, South Africa
Durban University of Technology, South Africa
University of Pretoria, South Africa
Posters & Accepted Abstracts: Drug Des
Background & Aim: Hepatocellular carcinoma is one of the leading global epidemics with highest incidence in developing countries such as South Africa. Traditional herbal medicines have been utilized for generations and a medicinal tree, Moringa oleifera (MO), have been part of a variety of treatments including cancer. We investigated the anti-proliferative and apoptosis inducing effects of MO crude aqueous leaf extract (MOE) in human liver hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cell viability was evaluated using the MTT assay. Oxidative stress and DNA damage was determined using the TBARS and comet assays respectively. Apoptosis was assessed by caspase-9, -3/7 activities and ATP levels (luminometry). Cell cycle, �?³H2AX and cleaved PARP-1 were determined by flow cytometry. Protein expression of c-myc, Bax, p-Bcl2, Smac/ DIABLO, Hsp70, SRp30a and cleaved PARP-1 was assessed using western blotting. Results: HepG2 cells were exposed to various concentrations of MOE for 24 h and an IC50 of 4.479 mg/ml was determined. MOE caused a significant increase in lipid peroxidation, DNA fragmentation and �?³H2AX levels. A significant decrease in G1, S and G2-M phase was seen with a concomitant increase in apoptosis. SRp30a protein expression was significantly increased which led to the alternate splicing and subsequent activation of caspase-9. Caspase-9 and -3/7 was significantly increased with a significant decrease in ATP levels. Apoptosis was further confirmed with the significant decrease in c-myc, p-Bcl2 and Hsp70 protein expression and a significant increase in Bax, Smac/DIABLO and cleavage of PARP-1. Conclusion: MOE induces cell cycle arrest, alternate splicing and apoptosis in cancerous HepG2 cells.
Charlette Tiloke has completed her PhD at the Department of Medical Biochemistry at University of KwaZulu-Natal and is currently a Post-doctoral Research Fellow. Her research interests include anticancer and antimicrobial activity of medicinal plants and their synthesized nanoparticles.
Email: charlettetiloke@gmail.com