Molecular determination of multi-drug resistant Mycobacterium tub | 33440
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Molecular determination of multi-drug resistant Mycobacterium tuberculosis among HIV infected patients visiting tertiary care hospital, Nepal

6th International Conference on Structural Biology

August 22-23, 2016 New Orleans, USA

Amrit Lama Lo, Alina Maharjan, Ravi Bhakta Sherstha and Ranjeeta Shah

Pokhara University, Nepal

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Tuberculosis is serious and common opportunistic infection among human immunodeficiency virus (HIV) infected person and emergence of multi-drug resistant tuberculosis (MDR-TB) is growing threat to them due to suppressed immunity. Early detection of disease is an important task. Conventional detection methods are time consuming as compared to molecular detection methods i.e., Polymerase Chain Reaction (PCR). PCR is rapid diagnostic test. The aim of our study is to detect the Mycobacterium tuberculosis specific gene IS6110 and MPB64. Furthermore, to detect mutated rpoB gene for MDR-TB confirmation. 40 sputum specimens were collected from HIV infected patients either having symptom of tuberculosis or not enrolled in National Public Health Laboratory, Kathmandu, Nepal. All collected sputum were decontaminated with Modified Petroff��?s method then subjected to AFB staining and molecular detection method (i.e., Multiplex PCR) for detection of MTB co-infection, furthermore, allele specific PCR was done to detect MDR-TB. Only 7.5% (n=3) found to be smear positive whereas 92.5% (n=37) negative. Both smear positive and negative were further processed for MTB detection with Multiplex PCR and MTB positive further introduced to detect MDR-TB with allele specific PCR. Molecular method showed 12.5% (n=5) were TB co-infection. 60% MDR-TB had been detected among TB positive HIV patients. The prevalence rate of MTB and MDR-TB disease among HIV patients is much higher. The higher rate of MDR-TB is due to limitation in our study. Furthermore, we found that the proportion of HIV/TB co-infection is higher in male than female especially among economically productive age group.

Biography :