Rheumatology: Current Research
Open Access

Modulation of collagen-induced arthritis by a natural product resveratrol

International Conference and Exhibition on Orthopedics & Rheumatology

August 13-15, 2012 Hilton Chicago/Northbrook, USA

Damo Xu

Accepted Abstracts: Rheumatology & Orthopedics

Abstract :

RA is an inflammatory disease with unmet clinical need. Resveratrol, a safe, well-described plant-derived compound, possesses anti-inflammation and immune-regulatory properties in several experimental autoimmune diseases including diabetes, encephalomyelitis (EAE) and colitis. We therefore evaluate the therapeutic effect of resveratrol on collagen-induced arthritis (CIA) and its putative immune modulation in mice. CIA was induced in DBA1 mice by immunization with collagen II. Different doses of resveratrol were administered before or after the development of CIA. The levels of antibody and cytokines in serum or in draining lymph node (DLN) lymphocyte culture supernatants were measured by ELISA and Th17 cell development in DLN was monitored by flow cytometry. Either prophyllactic or therapeutic administration of resveratrol attenuated clinical parameters and bone erosion in CIA mice. The arthritis-protective effects were associated with markedly reduced serum levels of pro-inflammatory cytokines and collagen-specific, but not total, IgG, and with reduced numbers of Th17 cells and the production of IL-17 in DLNs. Furthermore, resveratrol was able to inhibit aryl hydrocarbon receptor (AhR) agonist-mediated Th17 cell polarisation in vitro. Thus, resveratrol modulates inflammatory arthritis in rodents by selectively suppressing key cellular and humoral responses necessary for disease development. This may in part explain the protective effects of red wine but importantly may offer a novel, effective and safe pathway whereby novel agents could be developed to treat RA.