Metabolism of gama-tocopherol in the liver of male Sprague-dawley | 44963
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Metabolism of gama-tocopherol in the liver of male Sprague-dawley rat (Rattus norvegiens)

13th International Conference on Structural and Molecular Biology: Techniques & Market Analysis

October 22-23, 2018 | Ottawa, Canada

Zhaokun Ding, Youqing Xu and Jhorn Gorge

Guangxi University, China

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Vitamin E (VE) plays important roles in the physiological and biochemical processes of animals and humans. However, VE is a unique vitamin that has not been understood thoroughly because the metabolites of VE are very complicated and hard to be analyzed. This experiment was conducted to study the metabolism of γ-tocopherol, one of 8 VE isomers, in the liver postmitochondrial supernatant (S9) of male Sprague-Dawley rat (Rattus norvegiens, SD-rat) using a novel approach. The results showed that an optimal reactive system of γ-tocopherol metabolism was composed of 5 mg/ml of S9, 8 μg/ml of γ-tocopherol, 1 mmol/ml of nicotine amide adenine dinucleotide phosphate (NADPH) and 45 min of reactive time at 35°C. The metabolites of γ-tocopherol could be successfully analyzed using gradient elution and reverse-phase high-performance liquid chromatography (RP-HPLC). The main metabolite was identified as �?-hydroxylate, a side chain of γ-tocopherol when the structure of γ-tocopherol metabolites was determined using gas chromatography-mass spectrometry (GC-MS). A conclusion was drawn that �?-hydroxylate, a main metabolite of γ-tocopherol, could be most utilized by SD-rat and other mammals.

Biography :