Izabela Kiluk, P Samczuk, R Bujak, A Kretowski, M Ciborowski and B Sobkowicz
Medical University of Bialystok, Poland
Medical University of Gdansk, Poland
Posters & Accepted Abstracts: J Clin Exp Cardiolog
Introduction: Pulmonary embolism (PE) is a major cause of mortality, morbidity, and hospitalization in Europe. In 30-40% cases of the PE, the cause (idiopathic or unprovoked PE) cannot be determined. Nowadays, metabolomics becomes important part of clinical studies. It can help to understand the disease process and propose diagnostic and prognostic biomarkers. Until, now, there was no study using untargeted metabolomics in population of acute PE patients. Purpose: The purpose of the study is to apply metabolomics to evaluate prognosis factors and identify novel diagnostic and prognostic markers for PE, what can also bring new insight into disease pathophysiology. Methods: Prospective analysis of plasma samples collected from 42 patients hospitalized in Cardiology Department (proven acute PE, treated with low molecular weight heparin (LMWH)) and 13 healthy controls treated with adequate dose of LMWH was performed. Samples were fingerprinted with LC-QTOF-MS. Differences between patients├ó┬?┬? subgroups (all PE patients, control after drug (CAD), subgroups of idiopathic PE patients with and without deep vein thrombosis (DVT)) and controls were evaluated. Depending on data distribution t-test or Mann-Whitney test were used. Results: Performed metabolomics analysis resulted in detection of more than 200 significantly altered metabolites. Multivariate statistics confirmed clear separation between the examined groups. Venn diagrams allow selecting common and differentiating compounds for particular comparison (Figure1). Significant changes were observed in level of cholesterol derivatives, acylcarnitines, phospholipids, fatty acid (like arachidonic acid) and their amides. Conclusions: Metabolic fingerprinting could be helpful to improve diagnosis and to understand pathophysiological mechanisms related to acute PE. The metabolites significantly different in acute PE patients were associated with hypoxia, lipid-related energy imbalance, alterations in mitochondrial function and signal transduction pathways. Moreover, some of the detected compounds were linked with cardiovascular diseases before.
Izabela Kiluk is a very promising Young Doctor, who started training in Cardiology in 01.01.2013 and PhD studies in 01.10.2013. Currently, she works as Resident Physician in the Department of Cardiology, Medical University of Bialystok Clinical Hospital. Since 4th year of the studies at the Medical University of Bialystok, she has been particularly interested in Cardiology and exhibited considerable part in the activities of Student’s Scientific Group of Department of Cardiology. During this time, she has proven to be a hardworking and dedicated Student, Scientist and now Physician, who would benefit from participation in such world-renowned event. She is the author of several publications concerning acute pulmonary embolism.