Mechanisms and modes of lead action on SOD inactivation in zebraf | 52565
Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 1478 350008

Mechanisms and modes of lead action on SOD inactivation in zebrafish livers

4th Global Summit on Toxicology

August 24-26, 2015 Philadelphia, USA

Rutao Liu and Hao Zhang

Scientific Tracks Abstracts: J Clin Toxicol

Abstract :

Lead toxicity has been proved to be related with inducing oxidative stress of organisms, and causing inactivation of antioxidant
enzymes, the mechanism of which remains unknown. This study investigated and compared superoxide dismutase (Cu/Zn SOD)
activity inhibited in lead-treated zebrafish livers and explored the mechanism of SOD inactivation by lead at the molecular level using
multiple spectroscopic techniques, isothermal titration calorimetric (ITC) measurement, molecular docking study and ICP-AES
detection. Results showed lead exposure decreased SOD activities in zebrafish livers due to direct interactions between lead and SOD,
resulting in conformational and functional changes of the enzyme. To be specific, studies at the molecular level indicated that lead
bound into the active site channel of SOD, hindered the path of the catalytic substrate (O2-·), damaged its skeleton conformation and
secondary structure, and interacted with the enzymatically related residue (Arg 141) through electrostatic forces (ΔH<0, ΔS>0), and
caused the release of Cu2+ and Zn2+ from the catalytic pocket of SOD. This work shows a correlation between results on organismal
and molecular levels, and obtains a possible model hypothesizing mechanisms of lead toxicity using in vitro experiments instead of
in vivo ones.

Biography :

Rutao Liu has completed his PhD from Shandong University and Postdoctoral studies from Albert Einstein College of Medicine, Yeshiva University. He is the Director of
China - America CRC for Environment & Health of Shandong Province. He has published more than 120 papers in reputed journals and has been serving as an Editor of
Advances in Environmental Protection.