Laboratory induced estradiol exposure stimulates transcriptional | 52544
Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 1478 350008

Laboratory induced estradiol exposure stimulates transcriptional responses in the sea anemone Exaiptasia pallida

4th Global Summit on Toxicology

August 24-26, 2015 Philadelphia, USA

Michael B Morgan and James Ross

Scientific Tracks Abstracts: J Clin Toxicol

Abstract :

Cnidarians do not have endocrine organs; however they are capable of responding to signaling molecules such as hormones.
Endocrine disruption has been suspected in cnidarians but no direct hormone interaction has been identified. Regulatory
pathways associated with hormone bio synthesis and signaling are essentially uncharacterized in cnidarians. Representational
Difference Analysis (RDA) is a differential gene expression technique that can successfully be applied to cnidarians experiencing
stress. The objective of this study was to isolate transcripts that were responsive to a sublethal exposure of estradiol. The sea anemone
Exaiptasia pallida was exposed to 20 μg/l estradiol for four hours. Results identify transcripts that appear to have functional
significance related to steroid exposure. Results presented also demonstrate how small labs with limited financial resources can
use RDA coupled with Quantitative Real-Time PCR (qPCR) to perform transcriptional analyses in hypothesis driven experiments
to identify potentially important biomarkers of stressor-specific exposures. Conclusions will discuss how human pharmaceuticals
through sewage treatment effluent are representative of a class of anthropogenic stressors capable of impacting aquatic invertebrates.

Biography :

Michael B Morgan completed his PhD at Georgia Institute of Technology. His Postdoctoral training included a position as a Postdoctoral fellow at Georgia Tech followed by
a position as a Postdoctoral Associate at Georgia State University. He is currently an Associate Professor of Biology at Berry College. His research interests are in stress
responses of aquatic invertebrates. Specifically he investigates how cnidarians respond to various types of anthropogenic stressors. His molecular expertise is in using
differential gene expression techniques to detect, isolate, and characterize gene transcripts expressed from corals experiencing stress.