Journal of Pharmaceutical Care & Health Systems
Open Access
ISSN: 2376-0419
ISSN: 2376-0419
A M Krupanidhi1, Prakash Dabadi1, Anand Hiremath1 and U Sreenivas2
ScientificTracks Abstracts-Workshop: J Pharma Care Health Sys
Objectives: Isolation, characterization and anti-neoplastic and antioxidant activities of active moiety from the Justicia beddomei leaves. Materials & Methods: The ethanol extract of the Justicia beddomei (JB) leaves has been purified by column chromatography. Elution was carried out with solvent mixtures of increasing polarities. Two compounds have been isolated from the obtained fractions. The structures have been confirmed by IR, 1HNMR, 13CNMR and Mass spectral studies. On the basis of spectral details the structures of the isolated compounds have been confirmed new compound-JB1designated as triterpenoid trisacharide, and compound �??JB2 named as Glucopyranosyl �?? (1-4) �?? glycopyranosy 1 �?? 3-0-24 hydroxy�? amyrin. The extracts and isolated constituents have been screened for anticancer and antioxidant activities in Swiss mice at the dose of 200 mg/kg (p.o). The anticancer activity of ethanolic, chloroform extract and fractions of chloroform extract was tested against Ehrlich�??s ascites carcinoma (EAC) induced tumours in Swiss mice. Treatment efficiency was assessed in terms of different extracts and isolated fractions of effects on the tumour volumes of the tumour beard mice relative to the control group. The two parameters of Specific tumour growth delay (SGD) and percentage of life span (ILS %) was calculated. The present investigation was also under taken to appraise properties of antioxidants on Ehrlich�??s Ascites tumour (EAS) induced mice. The total antioxidant activity of ethanolic extract, chloroform extracts and isolated fractions of JB1 and JB2 was estimated by 2, 2- diphenyl 1- picrylhydrazyl (DPPH) radical scavenging assay and superoxide dismutase (SOD) method. The DPPH scavenging potential of the ethanolic extract of Justicia beddomei ranged from 3.86 % to 81.46 %. The isolated fractions of Justicia beddomei of JB1 and JB2 showed percentage of scavenging ranged from 11.21 % to 57.40 % and -6.0 % to 84.55 % respectively. As per SOD of nitrobluetetrazolium method (NBT) IC50 was determined by spectroscopic method. Results: Administration of ethanol, chloroform extracts and isolated fractions of Justicia Beddomei leaves showed significant anticancer activity dose. The SGD of ethanolic and chloroform extracts and isolated fractions of JB was -0.83, -0.64 and -0.80 respectively. Similarly the percentage of life span was 57.14%, 57.14%, and 52.38% respectively. The results of present study have revealed that the chloroform extract and JB2 fraction showed significant antitumor activity. The local Ayurvedic Pandits were prescribed the aerial parts of JB leaves for treatment of tumour and abnormal growth. Based on this evidence the present study was to focus to evaluate the anticancer activity of ethanolic, chloroform and isolated fractions of Justicia beddomei leaves in albino mice. The experimental animals were injected with 106 Ehrlich�??s ascites carcinoma (EAC) cells S.C and extracts were administered orally. Administration of extracts to mice challenged with EAC showed significant anticancer activity. The observed anticancer activity of extracts was dose dependent. There was a significant increase in percentage of life span i.e., 57.14% in chloroform extract treated mice. The ethanolic and chloroform extracts was not toxic to mice even at the dose of 200 mg/kg body weight. The antioxidant activity of various extracts and fractions of Justicia beddomei was estimated by in vivo and in vitro methods. DPPH scavenging assay: Ethanolic extract and chloroform extract and DVF1 and DVF2 possessed 81.46 %, 55.27 % and 84.55 % inhibition of DPPH radical respectively. In SOD method, the highest percentage of inhibition was found in ethanolic extract i.e. 33.13 % as compared with control group. Conclusion: The ethanolic extract of Justicia beddomei possess significant anticancer activity against EAC-cells and antioxidant activity in DPPH and SOD model as compared to other extracts.
A M Krupanidhi is currently working as an Assistant Professor in the Department of Pharmacology, Bapuji Pharmacy College, India. He has several years of experience starting as a Lecturer at S.C.S. College of Pharmacy and as a Principal at M.M.J. College of Pharmacy. His research areas of interests are synthetic organic chemistry, natural product chemistry and pharmacological screening. He has about 20 research publications and several health care articles in several national and international journals. He also guided students in completing their projects at their MPharma and PhD level. His research in PhD is on the �??Investigation of Dodonaea viscosa and some synthetic compounds for anti-fertility and other pharmacological activities�?�.