Vineeta Singh and C. K. M. Tripathi
Accepted Abstracts: Biochem & Pharmacol
A microbial strain showing antibacterial (extra cellular) and antifungal (intra cellular) activity was isolated from a pretreated soil sample collected from the agricultural field of northern India and was characterized as Streptomyces capoamus. From the fermented broth of the culture, three compounds showing antimicrobial activity against various Gram-positive and Gram-negative bacterial cultures and Mycobacterium tuberculosis H37Rv were purified and two of them were characterized as actinomycin V and actinomycin D. Actinomycin V was evaluated in vitro against M. tuberculosis H37Rv by BACTEC radiometric detection system at concentrations ranging from 50 to 1.56 μg ml-1. The MIC of actinomycin V, streptomycin and rifampicin for M. tuberculosis H37Rv were found to be 3.125, 2 and .075 μg ml-1 respectively. Besides above activities actinomycin V showed some promising activity against Trichomonas vaginalis with very low MIC value 10 μg ml-1. This is the first report to mention as Streptomyces capoamus producer of actinomycin V and D and antitubercular and antitrichomonas activity of actinomycin V.