Journal of Cell Science & Therapy
Open Access

Iron-containing nanoparticles for cell-mediated hyperthermia

International Conference on Emerging Cell Therapies

October 1-3, 2012 DoubleTree by Hilton Chicago-North Shore, USA

Stefan H. Bossmann

Scientific Tracks Abstracts: J Cell Sci Ther

Abstract :

U sing magnetic nanoparticles to absorb alternating magnetic field energy as a method of generating localized hyperthermia has been shown to be a potential cancer treatment. We are developing delivery systems that use tumor homing cells to actively carry iron/iron oxide nanoparticles into tumor tissue. We have developed strategies to change the ligand spheres of superparamagnetic iron/iron oxide nanoparticles to facilitate their rapid uptake by cells capable of ameboid movement. This approach comprises the synthesis of nanoparticles in microemulsions, the exchange of the nanoparticles? organic ligands after synthesis, and the formation of double-layers around an iron/iron oxide core (ferrosomes). In addition to making the nanoparticles sufficiently water soluble, we have been exploring uptake-enhancing peptide sequences, as well as the attachment of small molecules. To date, we have been testing tumor-tropic neural progenitor cells, endothelial precursor cells, monocyte/ macrophage-like cells and neutrophils. We have used murine models of disseminated peritoneal pancreatic cancer, metastatic melanoma, and metastatic breast cancer. After tumor development, the transport cells loaded with iron/iron oxide nanoparticles were injected either intraperitoneally or intravenously and then allowed to migrate into the tumor. The mice were then exposed to an alternating magnetic field for 20 minutes to cause the cell-delivered nanoparticles to generate heat. We could demonstrate that cell-mediated A/C-magnetic hyperthermia is a viable alternative to intratumoral injection or intravenous injection of nanoparticles. The advantage of cell- delivered methods is that the total amount of nanoparticle required is distinctly lower. Depending on the nature of the delivery cells, different sites within the tumor can be targeted

Biography :

Stephen Waldman is an Associate Professor and Canada Research Chair at Queen?s University. He has been jointly appointed between the departments of Chemical Engineering and Mechanical & Materials Engineering since 2003. His research program is centered on the development of functional orthopaedic tissues (cartilage, ligament and IVD) with specific focus on the effects of biochemical and biomechanical stimuli. He has published over 60 journal articles, serves on the editorial boards of three biomedical engineering journals, and has served on several grant panel review committees of the Canadian Institutes of Health Research in the areas of regenerative medicine and biomedical engineering.