Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X


Inter-domain communication through intrinsically disordered region (IDR) revealed through the ensemble structure analysis

9th International Conference on Structural Biology

September 18-20, 2017 Zurich, Switzerland

Shin-ichi Tate

Hiroshima University, Japan

Scientific Tracks Abstracts: J Proteomics Bioinform

Abstract :

The functionally relevant inter-domain communication between the domains linked by intrinsically disordered region was explored by NMR in combination with small angle X-ray scattering. Based on the ensemble structure analyses and the numerical simulations to reproduce the chemical shift changes along with the substrate concentration, we have demonstrated how the domains cooperate to enhance the protein function through the substantially dynamic spatial allocation of the domains. Pin1, a proline cis/trans isomerase, comprises two domains linked by 10-residue IDR, one is the substrate biding domain to recognize pSer/pThr-Pro motif and the other is the enzyme domain that rotates the Pro peptide bond in the motif. The enzyme domain shows very limited affinity to the substrate, but its binding ability was enhanced by two orders of magnitude in the presence of the substrate binding domain linked by IDR; in which the inter-domain fly-casting mechanism plays to keep the substrate bound to Pin1 by tossing and receiving the substrate between the domains, once the substrate in bound to either one of the domains. A new functional aspect of IDR will be addressed.

Biography :

Shin-ichi Tate has got his PhD degree from the University of Tokyo in 1993. He has the experience as a visiting Researcher at ETH (Prof. K Wühtrich) and NIH (Dr. A Bax). He has been a Professor in the Department of Mathematical and Life Sciences at Hiroshima since 2006. He is now the Director of the Research Center for the Mathematics on Chromatin live Dynamics (RcMcd), and the Dean of the School of Science in Hiroshima University.