Current Synthetic and Systems Biology
Open Access
ISSN: 2332-0737
+44-77-2385-9429
ISSN: 2332-0737
+44-77-2385-9429
Maryam Kamalipourazad, Mohsen Sharifi and Hassan Zare Maivan
Tarbiat Modares University, Iran
Posters & Accepted Abstracts: Curr Synthetic Sys Biol
Manipulation of cell culture media by elicitors is one of most important strategies of biotechnology to induce secondary metabolism for the production of valuable metabolites. Natural elicitors such as chitosan are exogenous biotic compounds extracted from arthropods exoskeleton and fungal cell walls often act as specific elicitors in a number of plant cell cultures for efficient induction of valuable medicinally secondary metabolites. In this investigation, inducing effect of chitosan on physiological, biochemical and molecular parameters were investigated in cell suspension cultures of Scrophularia striata Boiss. Cells were treated with 10 mg/L chitosan and harvested for 3, 5 and 7 days after elicitation. Luteolin (Lut) and Myricetin (Myr) quantified by highperformance liquid chromatography (HPLC). Cell samples were used to elucidate the expression level of phenylalanine ammonialyase (PAL) and p-coumarate 3-hydroxylase (C3H) genes by semi-quantitative RT-PCR. Following treatments of chitosan, the results showed that the cell growth and viability of cells was decreased as compared to control. In addition, chitosan increased Lut and Myr contents. Cells elicited with chitosan for 5 days yielded the highest amount of Lut (12.69-fold) and Myr (11.15-fold) compared to the control cells. The expression of PAL and C3H genes by chitosan was increased, reaching a peak at 5 days after treatment (2.2-fold and 2-fold higher than control cells, respectively). Chitosan up-regulates the production of Lut and Myr, by effecting on gene expression of flavonoids biosynthesis pathway.
Email: mkamalipourazad@yahoo.com