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In vitro cotyledon culture of Cucumis melo var. utilissimus- A me | 1794
Biochemistry & Pharmacology: Open Access

Biochemistry & Pharmacology: Open Access
Open Access

ISSN: 2167-0501

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In vitro cotyledon culture of Cucumis melo var. utilissimus- A medicinal important plant


International Conference on Pharmacognosy, Phytochemistry & Natural Products

October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

M. Venkateshwarlu

Accepted Abstracts: Biochem & Pharmacol

Abstract :

Recent developments in molecular biology and genetic transformation however, have made it possible to identify, isolate and transfer desirable genes in to sugarcane. In dry land agriculture, the altered plants showed increased production. Most of the recent research was made on tobacco, Alfalfa and Arabidopsis thaliana . Causing extensive cellular damage and inhibition of photosynthesis is a phenomenon called oxidative stress and is known as one of the major causes of plant damage as a result of environmental stresses. The explants cultured on regeneration media fortified with different vitamins, auxins and cytokinins (BAP, kinetin) in the concentrations of 0.5 and 3.0 mg/l. One of the regenerates showed some variation in the morphology of leaves which led to the estimation of nutritional of qualities of the fruits of regenerant in order to detect any different between the control, the normal regenerents and variant obtained. Cell maintained in vitro undergo a certain level of spontaneous genetic change, which may be induced or expressed by culture conditions. Variant cells may be induced to generate. Seedling was grown in vitro from sterilized seeds on the MS basal medium and explants like cotyledons. These cultures were kept for shoot regeneration at 26 � 1 0C and under 16/8 photo period, all plants were normal except one plant regenerated from cotyledon explants was variant. This variant had exceptionally large leaves as well as had variation in protein profile and nutritional status. The regenerated shoots were transferred to the rooting medium and rooted plants were finally established in potted in the green house. Impact of various growth regulators on tissue and isolated plant cells was studied by several workers and these substances were known to play an important role in cell metabolism, cell membrane synthesis besides growth and differentiation, of these growth substances auxins have proved to be an essential supplement for establishing successful culture of plant tissues. Auxins were found to exert their hormonal action through their effects on the nucleic acids and protein metabolism. It also found that auxins axert their effects, directly or indirectly on ?S? phase of DNA synthesis. Physical environment viz., state of medium, light, temperature, humidity, etc., besides source and size of explants are known to play an important role in the in vitro organogenesis.

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