In vitro assessment of ROS on motility of epididymal sperm of mal | 16897
Endocrinology & Metabolic Syndrome

Endocrinology & Metabolic Syndrome
Open Access

ISSN: 2161-1017

+44 1478 350008

In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol

3rd International Conference on Endocrinology

November 02-04, 2015 Atlanta, USA

Ansoumane Kourouma

Huazhong University of Science and Technology, China

Posters-Accepted Abstracts: Endocrinol Metab Syndr

Abstract :

The mechanism by which nonylphenol (NP) interferes with male infertility is not yet fully elucidated. NP was evaluated for its effects on epididymal sperm of adult male rats. Twenty four Sprague-Dawley (SD) rats were used as epididymal sperm donors. Previously rats were administrated with NP (0, 2, 10 and 50 mg/kg) body weight respectively in corn oil every forty-eight hours by intraperitoneal injection for 30 days. Computer assisted sperm analysis (CASA) was used to determine parameters of sperm. The sperm morphology examination was conducted with a high resolution microscope. Results indicated that exposure to NP no effect on body weight, while testes weights were significantly decreased. Computer assisted sperm analysis (CASA) showed significant decline in the percentage of motile spermatozoa (P < 0.001), STR and LIN (P < 0.01), significant increase in ALH (P < 0.001), while significant decline in BCF (P < 0.001) respectively. Plasma LDH was significantly increased while; plasma �?³-GT activity was significantly decreased. H2O2 production and malondialdehyde (MDA) were significantly increased. The Plasma CAT, GSH-Px and SOD activities were significantly decreased. This concludes that NP leads oxidative stress in the epididymal sperm of rats. Moreover, NP can disrupt sperm motility and alterations in the sperm morphology.

Biography :