Mazumder Avijit, Mazumder Rupa and K. Nagarajan
Accepted Abstracts: Biochem & Pharmacol
The methanol extract of leaves of Cestrum nocturnum was tested for its antimicrobial activity against various pathogenic strains of E. coli, V. cholerae, Shigella dysenteriae, Salmonella typhi, Salmonella paratyphi A and Salmonella paratyphi B using both agar diffusion and broth dilution methods. The methanol showed considerable activities against S. typhi (24, 23 and 25 mm, respectively), S. paratyphi A (24, 24 and 25 mm, respectively) and S. paratyphi B (24, 24 and 26 mm, respectively). The hexane extract and aqueous extract did not show any significant activity on the tested bacteria strains. Minimum inhibitory concentration (MIC) varied from 1.25 to 2.5 mg/ml against different strains similar results were obtained for other tested strains too. The extracts proved to be bactericidal against all microbes except S. typhi and S. paratyphi A. The methanol extract was also screened against some multidrug resistant (MDR) clinical isolates too where results were very promising. Flavonoids, anthraquinones, triterpenes, saponins and tannins were found to be present, whereas alkaloids were not detected. Thus the results obtained suggested that methanol extract of leaves of Cestrum nocturnum has maximum in vitro antimicrobial activity against the causative agents of typhoid and paratyphoid fevers. It can be used alone or in synergism to combat drug resistance. However, in vivo study (i.e. antityphoid study) and sub-chronic toxicity study should be done further to ascertain the antityphoid action and determine the effect of the extract at the level of tissues and organs.