Journal of Glycobiology
Open Access
ISSN: 2168-958X
ISSN: 2168-958X
Ariadne Stavare
Posters-Accepted Abstracts: J Glycobiol
In this study the aim was to investigate the impact of the metoclopramide-induced hyperprolactinaemia on the apoptotic
and proliferation in the lacrimal gland of female mice. Then, 20 female/groups: control group (non pregnant, Ctr1): 0.2 mL
of saline (vehicle) and the experimental group (non pregnant, HPrl1): 200 �?¼g/day of metoclopramide, dissolved in vehicle.
After 50 days 10 females of each group were placed for mating with males and continued to receive treatment. The females non
pregnant were euthanasia on 50th day (experimental I) and the females pregnant were euthanasia on 5.5th to 6.5th post-coital
day (Ctr2: control group and HPrl2: experimental group). The blood samples were collected for hormone measurements. The
lacrimal gland was processed for immunohistochemistry. The results were subjected to statistical test (p <0.05). And results
showed decreased immunoexpression of caspase 3 in the non pregnant controls compared to non-experimental group (p
<0.05). And increased cell proliferation (PCNA) in the pregnant control group compared to pregnant experimental group
(p <0.05). Serum prolactin levels were higher whereas the levels of estradiol and progesterone were lower in the animals that
received metoclopramide compared to controls. These results suggest that the metoclopramide-induced hyperprolactinemia
altered the cellular activity in lacrimal glands in non-pregnancy and pregnancy as a consequence of the imbalance between
the apoptotic cell death (caspase-3 cleaved) and the cellular proliferation (PCNA). It is hypothesized that this effect might be
related with decrease in the hormonal production of estrogen and progesterone.