Journal of Glycobiology
Open Access

Hyperprolactinaemia changes the hyaluronic acid amount in the lacrimal gland of female mice

Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Ariadne Stavare

Posters-Accepted Abstracts: J Glycobiol

Abstract :

In this study, the aim was to investigate the impact of the metoclopramide-induced hyperprolactinaemia about amount of
hyaluronic acid (HA) in the lacrimal gland of female mice. 20 adult female mice were randomly divided into two groups
with 10 animals each: non control group (CTR, 0.2 mL of saline solution) and experimental group (HPRL, 200 �?¼g/day of
metoclopramide). Treatments lasted for 50 consecutive days. On the 50th, all animals were euthanized in proestrus phase and
lacrimal glands removed and blood collected. The lacrimal glands were processed for morphological analysis. The right gland
was placed in 10% buffered formaldehyde for processing immunohistochemistry and left lacrimal gland was placed in acetone
to the biochemical process for extracting and quantifying hyaluronic acid by fluorometric assay ELISA-like. The results were
submitted to statistical analysis with significance level �?±<0.05. The results showed by immunohistochemical analysis and the
biochemical analysis revealed significant decrease on amount of hyaluronic acid. These results suggest that hyperprolactinaemia
contributes to ocular dryness and it may lead to dry eye.