Drug Designing: Open Access
Open Access
ISSN: 2169-0138
ISSN: 2169-0138
Nayak Pratik P, Parmar Rajesh R and Dushyant A Shah
APMC College of Pharmaceutical Education and Research, India
Posters & Accepted Abstracts: Drug Des
In this study an HPTLC densitometric method was developed for the simultaneous determination of lamivudine and efavirenz in pharmaceutical dosage forms which are used widely as first line HIV/AIDS treatment. Chromatographic separation of the drugs was performed on precoated silica gel 60F 254 (0.2 mm thickness) plates using chloroform: methanol (9:1, v/v) as a mobile phase. A TLC scanner set at 255 nm was used for the direct evaluation of the chromatogram in reflectance absorbance mode.The two drugs were satisfactorily resolved with Rf values of 0.12 �± 0.03, and 0.76 �± 0.04 for lamivudine and efavirenz respectively. Calibration curves were in the range 100-500 ng/band for lamivudine and from 300 - 1500 ng/band for efavirenz Correlation coefficients (r) values were 0.9988 for lamivudine and 0.9985 for efavirenz. A low relative standard deviation (< 2%) was found for both precision and robustness study showing that the proposed method was precise and robust. The method had an accuracy of 99.34% and 99.37%; and percentage assay of 99.52% and 100.21% for lamivudine and efavirenz respectively. Method had the potential to determine these drugs simultaneously from dosage forms without any interference.