Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Balko J
Charles University, Czech Republic
Scientific Tracks Abstracts: Gynecol Obstet
Introduction: Uterus transplantation (UTx) is a causal treatment for absolute uterine factor infertility (AUFI). Assessing rejection signs using a histopathological examination of the ectocervical biopsy from the transplanted uterus is a common practice in all human UTx worldwide to date. A provisional scoring system was used for the histopathological assessment of subclinical rejection signs in uterus recipients. Here we hypothesized that histopathological and immunohistochemical f indings in the normal uteri would differ from the borderline cat ego ry of subclinical rejection in uterine transplants. This finding would justify the existence of the borderline category and possible treatment of it. Methods: This prospective observational study included ectocervical biopsies of 54 women who underwent hysterectomy for benign reasons. All biopsy samples were assessed histopathologically and immunohistochemically (IHC) using CD45RO, CD8, CD4, CD20 and C4d antibodies. A semi quantitative scoring system was used to characterize lymphocytic populations and it was compared to the provisional grading system. Conclusion: The histopathological and immunohistochemical findings in the uteri of premenopausal women were similar to the borderline category of the currently used provisional scoring system of subclinical uterine rejection utilized in all uterine transplant studies. Therefore, the borderline category could actually represent a normal finding and no immunosuppressive treatment against rejection should be needed in such cases.
Dr. Jan Balko is a pathologist at the Department of Pathology and Molecular Medicine, Second Faculty of Medicine and University Hospital Motol, Charles University in Prague, Czech Republic. He has been serving in this position since August 2013, contributing to both diagnostic pathology and medical research. His professional interests in clude molecular diagnostics, surgical pathology, and translational research within oncology. Dr. Balko is actively involved in academic teaching and scientific collaboration, supporting the development of modern pathology prac t ices within the institution and the broader medical community.