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Hippocampal neural proteins interaction of Alzheimerandprime;s peptide: 14-3-3 zeta as potential therapeutic biomarker
Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Hippocampal neural proteins interaction of Alzheimer′s peptide: 14-3-3 zeta as potential therapeutic biomarker


Joint Event on 9th International Conference and Expo on Proteomics and Molecular Medicine & 9th International Conference on Bioinformatics

November 13-15, 2017 Paris, France

Pratibha Sharma and A Srinivasan

All India Institute of Medical Sciences, India

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Introduction: Alzheimerâ??s disease (AD) is the most common form of dementia. Amyloid beta(1-42) is prone to aggregate and found in plaque interacting with various proteins. Low molecular weight oligomeric form of amyloid beta is found as most toxic and responsible for disease process. Hippocampus is the primary region of the brain affected by AD. In this study, interaction profiles of oligomer and monomer form of amyloid-beta binding hippocampal neuron intracellular and cytosolic proteins were obtained. Methods: Amyloid beta(1-42) peptide- monomer and oligomer forms separated using gel-filtration chromatography. These were allowed to pull-down separately with hippocampal tissue neuron plasma membrane and cytosolic proteins using affinity chromatography in proteins native form. Interacting proteins were digested in-solution by trypsin and identified using mass spectrometry ESI-Triple-TOF5600, searched Uniprot database using software Protein pilot 4.2. Gene ontology and pathway analysis software Cytoscape was used for classification and interactions of proteins with respect to AD and apoptosis pathways. Result: Proteins found unique binding amyloid beta peptide monomer are synuclein-beta, SNAP25, lipophilin, EAAT-2 and SRPRB. Proteins found unique binding amyloid beta peptide oligomer are tubulin-beta, Tau(MAPT), 14-3-3 and phospholipase A2. Conclusion: Oligomer binding proteins may help in understanding the disease toxicity mechanism. Whereas, 14-3-3 protein could be possible novel therapeutic target for diagnosis, treatment and in understanding progression of AD.

Biography :

Pratibha Sharma is a PhD student at All India Institute of Medical Sciences, India.

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