Sleman Kadan, Yoel Sasson and Hilal Zaid
Qasemi Research Center, Al-Qasemi Academic College, Israel
The Hebrew University of Jerusalem, Israel
Scientific Tracks Abstracts: Biochem Pharmacol (Los Angel)
In the present in vitro study, we tested the chemical composition, cytotoxicity and anti-diabetic activity of two Wild Artichoke-like Vegetable, Gundelia tournefortii, distinct extracts: Methanol and hexane. GC/MS phytochemical analysis of Gundelia T. methanol and hexane extracts revealed 44 compounds reported here for the first time in Gundelia T. out of the 50 detected compounds. Only Stigmasterol was present in both extracts. The efficacy of Gundelia T. extracts in enhancing glucose transporter-4 (GLUT4) translocation (fig. 1) to the plasma membrane (PM) was tested in L6 muscle cells stably expressing myc-tagged GLUT4 (L6-GLUT4myc) using cell-ELISA test. Results obtained here indicate that methanol and hexane extracts where safe up to 250 �?¼g/ml as measured with MTT and the LDH leakage assays. The methanol extract was the most efficient in GLUT4 translocation enhancement. It increased GLUT4 translocation at 63�?¼g/ml by 1.5 and 2 folds relative to the control in the absence and presence of insulin, respectively. These findings indicate that Gundelia T. possesses anti-diabetic activity in part by enhancing GLUT4 translocation to the PM in skeletal muscle.
Sleman Kedan is a PhD student at Al-Qasemi research center, Al-Qasemi Academic College, Baka Algharbiya and Casali Institute for Applied Chemistry, The Hebrew University of Jerusalem, Israel where he finished his M.Sc in chemistry on 2010. His PhD thesis title is “Novel Anti-Diabetic Natural Drug Candidates: Isolation, Identification and Characterization of the Chemical Structures and Biomolecular Mechanisms”. Sleman have so far published 4 refereed papers, 3 conference abstarcts and is a coauthor of scientific book.