Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495


Genotoxicity of gamma rays in liver, lungs and lymphocytes of albino mice

6th Global Summit on Toxicology & Applied Pharmacology

October 17-19, 2016 Houston, USA

Tayyaba Ali, Zarina Iqbal, Muhammad Ismail and Farkhanda Asad

Government College University, Pakistan

Posters & Accepted Abstracts: J Clinic Toxicol

Abstract :

Radiation is a process that allows movement of energy from one place to other. In the last many years use of different types of ionizing radiations in diagnosis and treatment of various diseases has been increased. These radiations contain enormous amount of energy. When they strike on any living organism they penetrate into that organism crossing cell membrane and ultimately they reach the DNA causing damage to the DNA. Contact to ionizing radiation can cause damage to living tissues, which can result in mutation and some mutations may lead to cancer. A study was designed to detect and compare the impact of Gamma Rays on the liver, lungs and peripheral lymphocytes of the albino mice. Single cell gel electrophoresis assay commonly known as comet assay was used to detect the damage to the DNA. Albino mice were used in the study and were randomly divided into four groups and named as G1, G2, G3 and C. Each group contains 10 animals. G1, G2 and G3 were exposed to 1cGy, 3cGy and 5cGy of Gamma rays respectively for 1min. Group C was not exposed with any radiations and it served as negative control group. Immediately after exposure to gamma rays peripheral blood was isolated, mice were dissected; organs (liver and lungs) were minced and were subjected to comet assay. Significant damage (P<0.001) was observed in all three doses in liver, lungs and lymphocytes when compared with control. G1 and G2 were not significantly different from each other; however there was significantly higher DNA damage in G3 as compared to G1 and G2. Results of the present study indicate that even low doses of Gamma rays for short period are capable of causing DNA damage in blood and different organs of the mice. Damage further increases with increase in intensity.

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