Journal of Proteomics & Bioinformatics
Open Access

Genomic detection of New Delhi Metallo β-lactamase gene-1 (NDM-1) from hospital environment

6^th International Conference & Expo on Proteomics

March 29-31, 2016 Atlanta, USA

Varun C N and Raju Ravikumar

National Institute of Mental Health & Neurosciences, India

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

Hospital acquired infection represents a major problem in health care system. �?²-lactam class of antibiotics is commonly used for treating bacterial infections. The most common mode of resistance is through �?²-lactamase enzyme. New Delhi Metallo �?²-lactamase (NDM) is a class of �?²-lactamase enzyme causing resistance to �?²-lactam antibiotics. Previous study has reported the prevalence of NDM-1 gene in isolates from MDR-GNB (Multi drug resistant Gram negative bacilli) isolates. The current study aims to understand the presence of NDM gene in hospital environment. We collected swab samples from high risk wards including Deaddiction ward, neurology ward, neurosurgery ward, head injury ward, Pediatric neurology ward, Pediatric Neurosurgery ward, psychiatry ward and short stay ward. DNA was extracted using Cetyl trimethylammonium bromide-Sodium chloride method for maximum DNA recovery. The conventional PCR was standardized with forward primer 5â�?�?-GGGCAGTCGCTTCCAACGGT-3â�?�? and Reverse primer 5â�?�?-GTAGTGCTCAGTGTCGGCAT-3â�?�?. Of a total of 70 samples tested, 34 were found to be positive for NDM gene (48.57% positivity). Neurosurgery ward did not yield any positive results. Pediatric neurology ward tested positive for all the samples. Though the study does not identify the source organism harbouring the NDM gene, we have successfully identified key environmental areas which may serve as a source of spreading antibiotic resistance. By focusing on these key areas, it may be possible to reduce the spread of resistant bacterial infection.

Biography :

Varun C N is currently pursuing his PhD from Department of Neuromicrobiology, National Institute of Mental Health and Neurosciences (NIMHANS), India. His doctoral thesis involves studying Psycho-Neuroimmunological aspects of Schizophrenia.

Email: ravikumarbly@yahoo.co.uk