Generation and comprehensive characterization of induced pluripot | 41374
Journal of Cell Science & Therapy

Journal of Cell Science & Therapy
Open Access

ISSN: 2157-7013

Generation and comprehensive characterization of induced pluripotent stem cells generated with CTS Sendai viral reprogramming kit

10th World Congress and Expo on Cell & Stem Cell Research

March 19-21, 2018 | New York, USA

Uma Lakshmipathy

Thermo Fisher Scientific, USA

Posters & Accepted Abstracts: J Cell Sci Ther

Abstract :

The induced pluripotent stem cell (iPSC) field has grown immensely in the past ten years, starting with basic research, and now moving more toward clinical applications. As iPSC move towards the clinic, it is vital that high quality cells are used. These cells should be free of reprogramming transgenes, free of adventitious agents, genetically stable, and functionally pluripotent. Typical reprogramming workflows often include animal origin components, such as fetal bovine serum (FBS) or bovine serum albumin (BSA), which can be potential sources of adventitious agents. IPSC that are generated in a xeno-free, clinically relevant workflow will be free from any such potential contaminants, and much more readily applicable to clinical uses. Here, we show the use of a CTS Sendai reprogramming kit manufactured under GMP principles that is also free of animal origin components, to generate high quality iPSC. The kit was used to generate iPSC derived from both blood-derived cells, and skin fibroblasts. The reprogramming workflows used were xeno-free, starting from initial cell isolation, all the way through to iPSC expansion and banking. The iPSC generated with this method were further subjected to comprehensive characterization using traditional cellular methods and molecular methods such as PluriTest to confirm pluripotency based on gene signature and TaqMan hPSC ScoreCard to assess trilineage differentiation potential. The clones were further subjected to array based karyotype analysis at both low and high resolution using KaryoStat and KaryoStat HD, the combination of high quality reagents for consistent iPSC generation in xeno-free workflows, together with comprehensive characterization methods will allow researchers to develop robust protocols for basic research And a smoothly transition cells to clinical applications.