Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Abdul Ghaffar and Bushra Munir
Government College University, Pakistan
Posters-Accepted Abstracts: J Proteomics Bioinform
Phytic acid is an important constituent of poultry feed and is needed to be removed for efficient absorption of food ingredients from intestine. Phytases have got considerable importance in this field. A gene encoding phytase from newly isolated alkalophilic Bacillus pumilus SS2014 was cloned and expressed through pET32a into E. coli BL 21. The open reading frame consisted of 657 nucleotides which encoded 219 amino acids with molecular mass of 23 kDa. The cloning was carried out into pET-32a for its expression in E. coli BL21. The purified enzyme showed optimum activity at 60�?° C and pH 7.5. The Km was 1.28 �?¼mol and Vmax was 76 �?¼M per min. The enzyme was quiet stable at wide range of temperature describing its thermostability. The results showed that the phytase activity was increased by Na+, Mg2+, Zn2+, decreased by K+ and Cu2+ and totally suppressed by EDTA. The recombinant phytase showed much better characteristics as compared to wild type. Therefore, it has a potential for applications in broad spectrum of industry due to its alkalophilic and thermophilic stability.
Email: aghaffaruaf@yahoo.com