Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Keliani Bordin, Pamela Ruy, Estela Kobashigawa, Benjamim Landers, David Ledoux, George E. Rotthinghaus and Carlos Augusto Fernandes de Oliveira
Posters: J Clinic Toxicol
F umonisin B 1 (FB 1 ) is a common contaminant of corn products and is produced by fungi of the genus Fusarium . To date, only one research group has utilized the analysis of fumonisin B 1 in hair as a biomarker for the assessment of FB 1 exposure in animal models and in humans. It provides an important link in translating information to evaluate past fumonisin exposure. In the present study, an analytical method has been validated and tested for its applicability to measure FB 1 in hair. The hair samples were extracted with methanol, filtered, and the filtrate passed through immunoafinity columns followed by LC/MS analysis. The quantification limit of the method was 0.5 ng FB 1 /g hair. A standard curve was prepared with four concentrations of FB 1 ranging from 1 to 20 ng/mL with a correlation coefficient (r 2 ) of 0.997. As part of the validation, hair samples were collected from two groups of swine, one control group containing 1 mg FB 1 /kg and another group was fed 15 mg FB 1 /kg for 6 weeks. Hair samples of control group contained 4.7 ng FB 1 /g and fumonisin treated group contained 32.9 ng FB 1 /g. Human hair samples were collected individually from residents in Pirassununga, S�o Paulo State, Brazil. Initial results demonstrated that 25% of human hair samples were positive for FB 1 at levels 5 to 12.7 ng/g hair. These results suggest that there is an exposure to FB 1 from corn products.
Keliani Bordin holds a degree in Food Engineering from the University of Santa Catarina State, Brazil (2009). She is a PhD student at the University of S�o Paulo since 2010 evaluating biomarkers of fumonisins in human. She is currently an intern in the Veterinary Medical Diagnostic Laboratory at the University of Missouri, in Columbia, USA