Drug Designing: Open Access
Open Access

Evaluation of histopathologic and histomorphometric changes of adrenal gland tissue following consumption of methylphenidate in male mice

International Conference and Expo on Drug Discovery & Designing

August 11-13, 2015 Frankfurt, Germany

Simin Fazelipour1, Kaiei M1, Tootian Z2, Kiaei S.B1 and Gharahjeh M1

Scientific Tracks Abstracts: Drug Des

Abstract :

Background: One of the most common psychiatric disorders in children is ADHD (Attention Deficit Hyperactivity Disorder), which
is treated extensively by Methylphenidate. This study investigates the assessment of the effects of methylphenidate on histopathologic
and histomorphometric changes of adrenal gland and serum level of cortisol in male mice.
Material & Methods: In this study 30 adult male mice (Balb/C) were used. After determining the body weight, the animals were
divided randomly into two experimental groups and one control group. The experimental groups received methylphenidate via
gavage as follow: The group 1 received 2 mg/kg/day and the group 2 received 10 mg/kg/day for a period of forty days. After evaluation
of body weight, general anesthesia was used for taking blood samples from the heart in order to measure cortisol levels in serum.
After determining of body weight, Adrenal glands removed and its thickness of capsule, medulla and cortex were measured.
Results: The results showed that Methylphenidate with different doses could increase thickness of the glumerolosa and fasciculate
layer, and decrease of the reticularis layer and capsule thickness in experimental groups significantly. Besides some significant changes
in serum cortisol no significantly histopathological changes were seen in control specimens.
Conclusion: Our findings demonstrated that Methylphenidate administration in adulthood could affect cortisol hormones and
adrenal gland tissue.