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Evaluating protective effects of Asperugo procumbens L on hepatoc | 12634
Biochemistry & Pharmacology: Open Access

Biochemistry & Pharmacology: Open Access
Open Access

ISSN: 2167-0501

+44-20-4587-4809

Evaluating protective effects of Asperugo procumbens L on hepatocellular carcinoma in rats


Joint Event on 10th World Congress on Pharmacology & 6th International Conference and Exhibition on Advances in Chromatography & HPLC Techniques

August 02-03, 2018 | Barcelona, Spain

Mahsa Hosni

Zanjan University of Medical Sciences, Iran

Posters & Accepted Abstracts: Biochem Pharmacol (Los Angel)

Abstract :

Asperugo procumbens L. has been used in Iranian traditional medicine to thetreatment of liver diseases. This study evaluated the protective effects of the aqueous extract of Asperugo procumbents L. (AAP) against hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN) in male Sprague-Dawley rats. HCC was induced in male rats according to the accepted protocol. Briefly, DEN was used as the initiator and 2- Acetylaminofluorene (2-AAF) as the promoter of hepatocarcinogenesis. Firstly animals fasted 96 hours and then re-fed as mitotic proliferative stimuli. After 24 hours re-feeding, rats were injected a single intraperitoneal (i.p.) dose of DEN (200 mg/kg body weight). Two weeks after DEN injection, rats received 14 daily oral dose of 2-AAF (30 mg/kg) for promoting hepatocarcinogenesis. AAP-treated rats received the extract intragastrically at three dose of 100, 200 and400 mg/kg body weight two weeks before the administration of DEN and continued until 8weeks. A significant decrease in serum markers of liver damage and hepatic carcinoma,including alfa-fetoprotein (AFP), gamma glutamyltranspeptidase (GGT), alaninetransaminase (ALT), and aspartate transaminase (AST) were observed in AAP supplemented animals when compared to DEN-treated rats. In addition, AAP counteracted DEN-induced oxidative stress in rats illustrated by the restoration of reduced glutathione (GSH) and the reduction of malondialdehyde (MDA) levels in the liver. The results of morphological and histopathological staining of rat liver showed that AAP-treated animals have an almost normal histological architecture compared to HCC group. The present study provides evidence that Asperugo procumbents L. has apotential chemopreventive effect against liver cancer.

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