Biochemistry & Pharmacology: Open Access
Open Access

ETS1 is associated with cisplatinum resistance though IKKα/NF-κB pathway in MDA-MB-231

9^th World Congress on Pharmacology

September 04-06, 2017 | Paris, France

Yuzhu Zhang and Hongfeng Chen

Longhua Hospital Affiliated to Shanghai University of TCM, China

Scientific Tracks Abstracts: Biochem Pharmacol (Los Angel)

Abstract :

MDA-MB-231/DDP had higher IC50 value of DDP, lower intracellular DDP concentration, lower apoptosis ratio than MDAMB- 231 cell line treated with DDP. Considering the intracellular DDP concentration difference, we tested drug-resistant membrane proteins (MRP2, P-gp and BCRP), which were remarkably increased in MDA-MB-231/DDP cells. Next, we found these increased membrane proteins were induced by the activation of NF-�?ºB pathway in MDA-MB-231/DDP cells. Furthermore, ETS1, RPL6, RBBP8, BIRC2, PIK3A and RARS were six important genes for DDP-resistance based on PPI network and expression validation. However, it has been reported enforced over-expression of ETS1 induced IKK�?± mRNA and protein expression as well as IKK�?± promoter activity. Our results suggested the protein expression of ETS1 and IKK�?± were significantly up-regulated in MDAMB- 231/DDP cells. In addition, inhibition of ETS1 expression enhances chemo-sensitivity to DDP and reversed the activation of NF- �?ºB pathway in MDA-MB-231/DDP cells, whether ETS-1 binds to the core IKK�?± promoter and strongly induces its activity. Now, our team is researching the corresponding binding sites between ETS1 and IKK�?±by dual-luciferase and chromatin immunoprecipitation technique (ChIP).

Biography :

He is studying his PhD of medicine at Shanghai University of Traditional Chinese Medicine. His researches focus on key target genes of tumor prognosis, mechanisms of drug resistance and anti-cancer natural drugs.