Journal of Cell Science & Therapy

Journal of Cell Science & Therapy
Open Access

ISSN: 2157-7013

+44 1300 500008

Enhancing survival of transplanted stem cells in the wound bed


6th World Congress on Cell & Stem Cell Research

February 29-March 02, 2016 Philadelphia, USA

Alan Wells

University of Pittsburgh, USA

Scientific Tracks Abstracts: J Cell Sci Ther

Abstract :

Transplantation of stem cells to augment the intrinsic regenerative capacity is a potential therapy for non-healing wounds. However, such approaches have been less than successful, as the majority of introduced cells are lost within days due to cell death from the harsh wound environment that lacks nutrients and presents apoptosis inducing cytokines. A cellular engineering approach to enhanced survival could overcome this obstacle. Activation of the EGF receptor at the cell surface, but not from internalized receptors in the endosomes, promotes cell survival in the face of cellular starvation, death cytokine signaling and toxic agents. The signaling cascade involves tonic low-level activation of Erk MAPK and PI3K/Akt pathways. To achieve this mode of signaling the triggering ligand must be retained in or attached to the substratum or matrix. This can be accomplished by either tethering classical EGF receptor ligands to the substratum or activating the EGF receptor with cryptic ultralow affinity matrikines present in tenascin-C and laminin V. We have creating matrices with or without tethered EGF or tenascin-C. These were introduced into acute full thickness wounds in mice, internal soft tissue in mice (subfascial) and critical cortical bone defects in dogs. In all three instances wound healing and vascularization was significantly increased in the presence of EGF receptor ligand. Xenotransplanted stem cells were retained up to one month in immunocompetent mice, compared to less than a week without the tEGF or tenascin-C. The presence of these cells with the tenascin-C limited scarring in a hypertrophic scar model, even 6 months after wounding, when all the transplanted stem cells have been rejected. The extended survival of these transplanted stem cells allows them to educate the local wound environment and turn the healing towards regeneration and away from non-functional scarring or failure to heal. This represents a new approach to cellular support for dysrepair.

Biography :

Alan Wells is an Executive Vice-Chair and Thomas J Gill III Professor of the Departments of Pathology and Bioengineering. He holds an AB in Biochemistry from Brown University (1979), DMSc in Tumor Biology from the Karolinska Institute (1982) and MD from Brown University (1988). Following his studies, he has completed a Postdoctoral Fellowship in Tumor Biology at the University of California in San Francisco, California and a Residency in Laboratory Medicine at the University of California in San Diego, California. He has over 230 scholarly publications that have been cited over 15,000 times. These advances have been recognized by numerous grants, patents and invited keynote talks and professorships, as well as election to the American Society for Clinical Investigation and the American Association of University Pathologists (Pluto Club). He has consulted extensively to industry and has cofounded two companies.

Email: wellsa@upmc.edu

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