Current Synthetic and Systems Biology
Open Access
ISSN: 2332-0737
+44-77-2385-9429
ISSN: 2332-0737
+44-77-2385-9429
S Khamehchian, S Hosseinkhani, M Nikkhah and S Mohseni
Tarbiat Modares University, Iran
Posters & Accepted Abstracts: Curr Synthetic Sys Biol
Cell penetrating peptides (CPPs) have been developed as vectors for molecular delivery into various cells for use in drug delivery, gene therapy and cancer treatment by their property transporting various molecules into cytoplasm. CPPs with high internalization, cell specificity, and low cytotoxicity have been considered to increase the applicability for cell engineering. Gold nanoparticles (GNPs) are a useful tool for molecular imaging, because they are non-cytotoxic and have high solubility, ease of synthesis and excellent light scattering property. Here, we investigated the cell penetrability of TAT-C and MCaUF1-9(Ala)-C peptides conjugated to using of dark field imaging and atomic absorption spectroscopy. Depending on the peptide sequence had the different cell penetrating (CP) activity for three kinds of cell lines, Hela, A431, and MDA-MB-231. Peptide conjugated GNP showed low cytotoxicity and high selectivity against three cell types respect to net charge difference between peptide. MCaUF1��?9(Ala)-C-GNP and TAT-C-GNP displayed higher affinity for MDA-MB-231 (24.2%) and A431 (17.6%) cells, respectively. We studied the cytotoxic activity of an anti-cancer drug doxorubicin (DOX) conjugated to the peptide conjugated GNP. They showed different cytotoxicity against the three cell lines, depending on the peptide sequence, with a higher efficiency than free DOX at the same concentration. The cytotoxicity by DOX was correlated with the CP activity of the peptides against the three cell lines. DOX-MCaUF1��?9(Ala)-C-GNP induced the highest cytotoxicity in MDA-MB-231 cells (30%). These results demonstrated that peptide conjugated GNP would be a useful tool for the development of a new cell-selective drug delivery system.