Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Bakhtiyar Qader, Mark Baron, Issam Hussain and Jose Gonzalez Rodriguez
University of Lincoln, Brayford Pool, UK
Posters & Accepted Abstracts: J Clin Toxicol
A simple, rapid and sensitive electrochemical method for determination of Disulfoton and its metabolites (Disulfoton Sulfone) in a real sample was developed. The electrochemical behavior of Disulfoton and Disulfoton Sulfone by differential pulse voltammetry technique on a bare glassy carbon working electrode in a NaClO4 solution was investigated. An anodic peak current at potential 1136 mV for Disulfoton and 1285 mV for Disulfoton Sulfone were found. Oxidation peaks showed a linearity over a concentration range of 5-75 �?¼M (r2=0.9991) and 10-100 �?¼M (r2=0.9988) with lower limit of detection of 3.2 �?¼M and 1.73 �?¼M for Disulfoton and Disulfoton Sulfone, respectively. Intra-day and inter-day assay precisions, expressed as the relative standard deviation, were overall less than 7.54% for both analytes. The developed method showed a mean recovery percentage of 97.16% for Disulfoton and 97.54% for Disulfoton Sulfone in the prepared samples. The obtained recovery rates showed a good agreement comparing to those of recovered from GC-MS as a reference method. The proposed methods has been employed to determine Disulfoton and Disulfoton Sulfone in spiked plasma and urine samples with recovery ranging from 88.2% to 104.13% indicating that the developed methods can be applied for measuring the toxicant and its metabolite in a real sample.
Email: bqader@lincoln.ac.uk