Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
+44 1300 500008
ISSN: 2155-9880
+44 1300 500008
Young-Hyo Lim, Jun-Ho Joe, Ki- Seok Jang, Yi-Sun Song, Byung-Im So, Cheng-Hu Fang and Kyung-Soo Kim
Scientific Tracks Abstracts: JCEC
Purpose: We investigated eff ects of G-CSF on diabetic cardiomyopathy in sucrose fed Otsuka Long-Evans Tokushima Fatty (OLETF) rat. Methods: Seven-week old 20 male OLETF rats and 10 male Long-Evans Tokushima Otsuka rats were used as the experimental and control subjects. All of the LETO and 8 randomly selected OLETF rats were free access to tap water (OW) and other 12 OLETF rats were free access to sucrose contained water (OS). Aft er 10 weeks, each group was divided randomly into two groups and saline or recombinant human G-CSF (100 μg/kg/ day) was injected intraperitoneally for 5 days. Echocardiography was done just before treatment and sacrifi ce time. Results: In the Doppler echocardiography data, diastolic dysfunction was slow progression in the OG group. In the Masson Trichrom, the progression of perivascular and/or interstitial fi brosis is signifi cantly decreased in OG group. In the immunohistochemical fi ndings, the extent of TGF-β immunoreactivity in the interstitial and perivascular tissue is signifi cantly decreased in OG group. In the EM fi ndings, the ultrastructure of ON group myocardium displays intracellular edema, deposition of collagen fi bers in pericapillary region, swelling and/or disrupted cristae of mitochondria, damaged myofi laments and intracellular junctions, as well as increased accumulation of lipid droplets and lysosomes in cardiac cells. In contrast, OG group revealed absence of collagen deposition and relatively decreased number of abnormal mitochondria. Conclusions: Th e present study provides evidence of the functional and histological benefi cial eff ects of G-CSF on diabetic cardiomopathy in OLETF rats