Rheumatology: Current Research
Open Access

Dynamically hydrophilic nanosprings, a synthetic collagen bionanomaterial, promotes attachment of osteoblasts and fibronectin selectively over albumin

International Conference and Exhibition on Orthopedics & Rheumatology

August 13-15, 2012 Hilton Chicago/Northbrook, USA

Jamie Hass

Posters: Rheumatology & Orthopedics

Abstract :

O sseointegration is dependent on the surface interaction between the biomaterial and serum proteins immediately upon implantation, stimulation of osteogenic behaviors of osteoblasts, along with strong mechanical properties. The design of biomimetic materials that parallel the morphology and biology of bone extracellular matrix is key to the ability to grow functional tissues in vitro and to enhance the integration of biomaterial implants into existing tissues in vivo. We have already shown the ability of normal human osteoblasts to propagate, differentiate and promote mineralization on silicon dioxide nanosprings1, which have been fabricated on titanium and medical orthopedic plates and screws. Now, we tested different wettable nanospring surfaces for promoting attachment of osteoblasts, fibronectin and albumin. Utilizing surface science spectroscopy, biologic cellular staining techniques and surface microscopy to determine that a moderate hydrophilic dynamic nanospring surface stimulated attachment of osteoblasts by 100‐fold over extremely hydrophobic and hydrophilic surfaces and fibronectin adsorbed 3:1 over albumin, in a physiological protein solution, on this surface. Albumin preferred hydrophobicity and blocked cellular attachment on all surfaces, whereas fibronectin attachment was needed for filopodia extension. We have established that silicon dioxide nanosprings can be used as a biomimetic material paralleling the morphology and biology of osteogenic extracellular matrix and enhance normal human osteoblasts cellular behaviors needed for improving osseointegration of orthopedic materials. Osseointegration is also dependent on the surface interaction between the biomaterial and serum proteins immediately upon implantation. A dynamic wettable nanospring enhanced device would selectively adsorb fibronectin over albumin; stimulate osteoblast attachment and therefore clinically impacting orthopedic implantation success.

Biography :

Jamie Hass, D.V.M., M.S., received her bachelors? degrees from Colorado State University and University of Idaho; a master?s from the University of Idaho, and her D.V.M. from Colorado State University and is currently a Ph.D. Candidate in physics at the University of Idaho. She is the co-owner of Silver Valley Veterinary Clinic and Benewah Veterinary Clinic in North Idaho. Dr. Hass is the current Chief Staff Veterinarian for Northeastern Wildlife, an animal research facility involved with numerous pharmacokinetics, surgery, oncology, imaging, and hepatitis studies, in which she maintains the health of over 400 animals and oversees the human orientated studies. Her current research at the University of Idaho is condensed solid physics, specifically the application of nanosprings to enhance osseointegration of orthopedic devices. She is a recipient of Idea Network of Biomedical Research Excellence Fellowship and Idaho Innovation Award in Early Stage Technology. Dr. Hass has founded MJ3 Industries, LLC, a biomedical nanotechnology company