Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Joanna Niemiec, Agnieszka Adamczyk, Aleksandra Ambicka, Krzysztof Małecki, Wojciech M. Wysocki, Jerzy Mitus and Janusz Rys
Scientific Tracks Abstracts: J Proteomics Bioinform
The assessment of ER/PR/HER2 expression and MIB-1 labelling index (MIB-1 LI) in primary tumor are strongly recommended in breast cancer patients as prognostic and predictive factors. However, it remains controversial if the above-mentioned parameters can be used to predict sensitivity to chemotherapy. Among other markers with potentially prognostic/predictive value, lymphatic vessel density as well as CD44/CD24 expression are considered. Disease-free survival (DFS) of 102 invasive ductal breast cancer patients (T≥1, N≥1, M0), treated with radical mastectomy and adjuvant chemotherapy with anthracyclines and/or taxanes was analysed according to selected immunohistichemically assessed parameters: (i) MIB-1 labelling index (MIB-1 LI), (ii) distribution of podoplanin labelled vessels (DPV), as well as expression of (iii) basal markers (P-cadherin, cytokeratin 5/6 ? CK5/6, smooth muscle actin ? SMA), and (iv) CD44/CD24. We found significantly longer DFS in patients with tumors characterized by: (i) MIB-1 LI≤28% or expression of all basal markers (DFS 91% vs. 63% for MIB-1 LI>28% and basal markers negativity, p=0.001), (ii) lack of lymphatic vessels or high tumor DPV (DFS 80% vs.44% for DPV ranging from 1% to 50%, p=0.005), (iii) CD44+/CD24-(DFS 80% vs. 44% for other CD44/CD24 expression patterns, p=0.005). Cox multivariate analysis revealed that both low DPV and high proliferation index/basal markers negativity were significant negative prognostic factors (CD44/CD24 expression was at significance border). Our results suggest that these markers could be considered predictors of resistance to chemotherapy based on taxanes and/or anthracyclines.
Joanna Niemiec has completed her Ph.D. at the age of 32 years from Department of Applied Radiobiology, Maria Skłodowska-Curie Memorial Institute Cracow Branch, Poland (thesis: Prognostic significance of biological parameters in surgically treated squamous cell lung cancer patients). She is the assistant researcher. She has published more than 20 papers in reputed journals and was a project manager and principal investigator in grants of Polish Ministry of Higher Education and Science. Joanna Niemiec is a member of Polish Radiation Research Society, Polish Histochemical and Cytochemical Society, Polish Society of Oncology.