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Development of criteria for quality of medicinal herb Artemisia c | 7881
Drug Designing: Open Access

Drug Designing: Open Access
Open Access

ISSN: 2169-0138

+44 1223 790975

Development of criteria for quality of medicinal herb Artemisia cina


2nd International Conference and Expo on Drug Discovery & Designing

October 27-29, 2016 Rome, Italy

Bekezhanova Tolkyn, Mamatova A S, Nurbayuly A, Sakipova Z B, Omarova R A and Shukirbekova A B

Kazakh National Medical University, Republic of Kazakhstan

Posters & Accepted Abstracts: Drug Des

Abstract :

Introduction: The process of drug development should be aimed at creating a quality product, and includes a reasonable selection of criteria in compliance with the SPh. Scientific researches of Kazakhstani plants aiming at the exploring of the possibility of their use in medicine and pharmacy are being conducted in S D Asfendiyarov Kazakh National Medical University in cooperation with scientists of School of Pharmacy and Pharmaceutical Sciences, Trinity Sciences Biomedical Institute, and Trinity College (Dublin, Ireland). Particular attention is paid to the herb Artemisia cina, which is an endemic plant of Central Asia and Kazakhstan. A wide range of biologically active substances and insufficient knowledge about them as a potential object for creation medicinal Phytosubstances were the basis for a full-scale research being conducted by scientists of the above mentioned Pharmacy schools. Aim: Study and defining of pharmacopoeia quality parameters of herb Artemisia cina. Materials & Methods: The object of the study is the aerial part of herb Artemisia cina, collected in South Kazakhstan region in the month of July 2015 in the flowering stage. Collecting and harvesting of medicinal plant Artemisia cina (Artemisia cina Berg.) was carried out in accordance with Good practice of collecting medicinal plants (GACP). In accordance with the pharmacopoeial requirements the parameters of quality specification are: description, identification (external signs, microscopy, thin layer chromatography, high-quality reaction), impurities, loss on drying, total ash, ash insoluble in hydrochloric acid, microbiological purity, radio nuclides, heavy metals and quantification. Quantification of santonine in herb Artemisia cina was done by HPLC Waters 1525 (column 0.15 m�?�?4.6 mm) in a gradient mode (acetonitrile-water in the ratio of P 35:65, 45:55, 55:45, 65:35). Output of santonine was 2.5%. Results: The external sight of the plant has been defined in accordance with the requirements of the State Pharmacopoeia of Kazakhstan ("Determination of morphological groups of medicinal plants.") In the process of the microscopic analysis anatomical and diagnostic features of the upper epidermis, lower epidermis, the structure of the stomatal apparatus and other specific characteristics have been revealed. Santonine is detected in the thin layer of sorbent plates C18 Silica TLC Plates and in the solvent system of chloroform-methanol-water P (9: 1: 1) while viewing the chromatogram in the UV spectrum at a wave length of 254 nm as dominant spots of light brown color Rf about 0.67. The presence of essential oil was found when viewing stained green spots under the microscope using Sudan III dye. Standards ratios: impurities (maximum 1%), loss on drying (maximum 13.0%), total ash (maximum 9%), ash insoluble in hydrochloric acid (2%), radio nuclides (24Bq / kg) and heavy metals in compliance with the requirements for the quality of raw materials. Conclusions: As a result of the studies conducted a list of indicators in accordance with the requirements of SPh of the RK for the quality of medicinal herbs has been made; specification of the quality (Quality Specification) and a project of AND for herb Artemisia cina have been developed.

Biography :

Email: tolkyn1984@mail.ru

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