Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Momoh A. Yakubu and Naga V. Naidu
AcceptedAbstracts: J Clinic Toxicol
L indane (hexachlorocyclohexane) is a persistent environmental toxin and a known neurotoxin. High levels of lindane have been detected in postmortem human brain from Parkinson's disease patients. Metabolism of Lindane produces several metabolites which are distributed in the body and excreted through various routes like urine, sweats, feces, hairs etc. But the detection of lindane and metabolites are still imperfectly identified. Therefore, an easy, fast and sensitive method to detect lindane and metabolites is highly desirable. We have determined lindane and its metabolites in urine, serum and feces samples using HPLC-UV-Vis and mass characterized with MALDI-TOF. Serum, urine and feces samples were collected following treatment of rats with lindane (17.6 mg/Kg; 1/5 of LD 50 ) or vehicle orally for 4 weeks. Lindane and metabolites were extracted following treatment with hexane. The extracts were subjected to HPLC-UV-Vis analysis and confirmed with MALDI-TOF. HPLC spectrum of standard lindane peaks were determined and compared with that from urine, serum and feces samples. The HPLC spectra confirmed the presence of lindane and its metabolites at different concentrations in urine (386-1652ppm), serum (207-371ppm) and feces (5.76-73.77ppm. Control samples did not show any peak corresponding to lindane. MALDI-TOF analysis of standard lindane showed a peak corresponding to 293 m/z whereas the urine and serum samples showed a major peak and feces samples showed a minor peak at 292-293 m/z corresponding to lindane and its metabolites. The concentration of lindane and its metabolites in the feces were found to be very low compared to that detected from urine and serum samples. These results show that HPLC is sensitive for detecting lindane and its metabolites and MALDI-TOF can identify specific metabolites of Lindane in samples by matching spectra peaks generated to the molecular weights of the metabolites. It further shows that minor quantity of lindane and metabolites are excreted through feces confirming that the major pathway for excretion of lindane and metabolites is through urine. Thus, HPLC-UV-Vis-MALDI-TOF analysis can be a reliable, non-invasive method of detecting lindane burden in serum, excretion in urine and feces. The HPLC-MALDI-TOF is highly sensitive to the point of detecting very low level (5 ppm) of lindane and metabolites in biological samples