Coronary angiogenic effect of long term administration of Nigella | 53963
Clinical & Experimental Cardiology

Clinical & Experimental Cardiology
Open Access

ISSN: 2155-9880

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Coronary angiogenic effect of long term administration of Nigella sativa

11th Annual Cardiology Summit

September 12-13, 2016 Philadelphia, USA

Lubna Ibrahim Al Asoom

University of Dammam, Saudi Arabia

Posters & Accepted Abstracts: J Clin Exp Cardiolog

Abstract :

Background: Coronary angiogenesis was reported as one of the preferable cardiac adaptive responses of aerobic exercise training. Induction of coronary angiogenesis might provide a protective effect against ischemic cardiac insults. Previously, long term administration of Nigella sativa showed inotropic and hypertrophic cardiac effects. No previous studies explore the coronary angiogenetic effect of Nigella sativa. Therefore, in the current study, long term administration of Nigella sativa is compared to exercise training regarding the induction of coronary angiogenesis. Method: 20 Wistar adult male rats were divided into four groups: Control, Nigella fed, exercise trained, Nigella fed-exercise trained groups. 800 mg/Kg Nigella sativa was administered orally to the nigella-fed rats for 8 weeks. Exercise rats were trained on 5-lane treadmill with speed 18 m/min and grade 30�?º for one hour daily for 8 weeks. By the end of the experiment, hearts were extracted and immunohistological slides were prepared using rat vascular endothelial growth factor (VEGF), platelet endothelial cell adhesion molecule-1 (PECAM-1), Von Wellibrand factor (VWF) and nitric oxide synthase-2 (NOS-2) antibodies (Ab). Photomicrographs were analyzed using Image J software. Percentage Ab-labeled-area of ten fields per specimen was recorded. Results: Using analysis of variance and LSD post hoc test, VEGF was significantly higher in Nigella fed and exercise trained rats compared to the control (p<0.01). Von Wellibrand factor was significantly lower in all groups compared to controls (p<0.01). Exercise trained group only exhibited higher PECAM-1 (p<0.01) and lower NOS-2 (p<0.05), compared to controls. Conclusion: The present demonstrated increase in VEGF and decrease of VWF in Nigella fed and exercise trained rats might reflect the potentiality of induction of coronary angiogenesis by these two interventions. Therefore, Nigella sativa treatment might also provide novel protective strategy to cardiac ischemia in vulnerable subjects.

Biography :