Comparative study of antimicrobial guided assay and bioactive com | 32191
Journal of Pharmaceutical Care & Health Systems

Journal of Pharmaceutical Care & Health Systems
Open Access

ISSN: 2376-0419

+44 1300 500008

Comparative study of antimicrobial guided assay and bioactive component determination, in vitro antioxidant, minerals, anti-nutrient and proximate analysis of aqueous and ethanol extracts of Alchornea laxiofolia and Pili

4th African Pharma Congress

June 20-21, 2016 Cape Town, South Africa

Osuntokun Oludare Temitope

Adekunle Ajasin University, Nigeria

Posters & Accepted Abstracts: J Pharma Care Health Sys

Abstract :

Piliostigma reticulatum plant in West Africa is an evergreen shrub with a twisted bole to 9 m high, with a bushy spherical canopy, bark dark grey to brown, fibrous and corky, slash dark red, leaves large, thick, leathery, grey-green, 6-12 cm long, fruit large, long, straight, undulate or twisted and hard, either glabrous or sparsely pubescent, brown, indehiscent, up to 25 cm long, 5 cm wide. Alchonea laxifolia is a Deciduous, erect to straggling shrub 7 m tall. Two extracting solvents were used for extraction, (ethanol and aqueous). Six clinical and environmental organisms were used for this research work. The organisms are Escherichia coli, Salmonella Typhi, Candida albicans, Klebsiella pneumonia, Aspergilus flavus and Staphylococcus aureus. Agar well diffusion assay were used to determine the antimicrobial activities of the plants. Both plants exhibit different degree of antimicrobial zones inhibition. Quantitative and qualitative phytochemical screening was also determined. It was observed that both plants contain various bioactive components like alkaloids, cardiac glycoside, flavonoids, steroids, tannin, saponin and phenol. Quantitative analyses of minerals present were determined (ug/100 g), minerals like Na2+, k+, Ca2+, Mg2+, Zn2+, Pb and P+ were present at various percentage composition by mass. Quantitative analyses of anti-nutrients present were elucidated. Anti-nutrients like tannin phenol, oxalate, saponin and flavonoids were present in various quantities. Proximate analyses of both plants were determined. In vitro antioxidant activities of ethyl acetate leaf extracts of the plants were also determined. The purpose of this research work is to determine the antimicrobial activities, minerals present, anti-nutrients, proximate contents and in vitro antioxidants activities of Piliostigma reticulatum and Alchonea laxiofolia.

Biography :