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Comet assay with gill cells of Mytilus galloprovincialis endpoint | 51088
Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

Comet assay with gill cells of Mytilus galloprovincialis endpoints tools for biomonitoring of water antibiotic contamination: Biological treatment is a reliable process for detoxification


2nd International Summit on Toxicology

October 07-09, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Hedi Ben Mansour

Accepted Abstracts: J Clinic Toxicol

Abstract :

T his work investigates the ability of Pseudomonas peli to treat industrial pharmaceuticals wastewater (PW). LCMSMS analysis revealed the presence, in this PW, of a variety of antibiotics such as Sulfathiazole, Sulfamoxole, Norfloxacine, Cloxacilline, Doxycycline and Cefquinome. P. p e l i was very effective to grow in PW and induct a remarkable increase of chemical oxygen demand (COD) and Biochemical oxygen demand (BOD) (140.31% and 148.51% respectively). On the other hand, genotoxicity of the studied effluent, before and after 24h-shaking incubation with P. p e l i , was evaluated in vivo in the Mediterranean wild mussels Mytilus galloprovincialis using comet assay for quantification of DNA fragmentation. Results show that PW exhibited a statistically significant (P<0.001) genotoxic effect in dose dependent manner, indeed, the percentage of genotoxicity was 122.6 and 49.5% after exposure to 0.66 ml/kg bw; 0.33 ml/kg bw of PW, respectively. However, genotoxicity decreased strongly when tested the PW obtained after incubation with P. p e l i . We can conclude that genotoxicity, using Comet assay, endpoints are useful tools to biomonitor the physicochemical and biological quality of water. We can conclude that P. p e l i can treated and detoxify the studied PW

Biography :

Hedi Ben Mansour has completed his Ph.D. at age of 28 years from Caen-Bass Normandy French and postdoctoral studies from Caen University (IUT-Caen) and University habilitation from Manouba University - Tunisia. He is the director of ?Micropolluant & bioremediation? research unit in Sidi Thabet city Manouba University (Tunisia). He has published 1 book; more than 41 papers in reputed journals and has been serving as an editorial board member of repute. He has obtained International price for the for young francophone researchers (Mail 2013) discipline "Science and Medicine" Sao Paulo - Brazil.

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