Rheumatology: Current Research
Open Access

Chondroitin sulfate and baekjeol-tang regulate thymic stromal lymphopoietin production and caspase-1 activation through negative regulation of IL-32 pathways

International Conference and Exhibition on Orthopedics & Rheumatology

August 13-15, 2012 Hilton Chicago/Northbrook, USA

Hyun-Ja Jeong

Scientific Tracks Abstracts: Rheumatology & Orthopedics

Abstract :

I nterleukin-32 (IL-32) was discovered in the synovial of patients with rheumatoid arthritis (RA). Thymic stromal lymphopoietin (TSLP) is also associated with RA. The purpose of this study was to investigate the specific mechanism of between IL-32 and TSLP in RA. In this study, we showed that IL-32 highly increased TSLP production and expression in human monocyte cell line, THP-1 cells. IL-32 induced TSLP production via activation of caspase-1 and nuclear factor-�?ºB. Depletion of TSLP prevents differentiation of monocytes into macrophage-like cells. Chondroprotective drugs such as chondroitin sulfate (CS) and the traditional Korean medicine, BaekJeol-Tang (BT) decreased production of TSLP and activation of caspase-1 and nuclear factor-�?ºB. In addition, CS and BT inhibit IL-32-induced monocyte differentiation. Taken together, IL-32 and TSLP are important cytokines involved in the development of RA. The effects of CS and BT were associated with the down-regulation of TSLP and caspase-1 through negative regulation of IL-32 pathways in RA