Drug Designing: Open Access
Open Access

Chlorogenic acid: A potential molecule with β-lactamase inhibitory activity against drug resistant bacteria

International Conference and Expo on Drug Discovery & Designing

August 11-13, 2015 Frankfurt, Germany

Pallavi Sahare and Archana Moon

Posters-Accepted Abstracts: Drug Des

Abstract :

Bacterial infections have been the major cause of diseases throughout the history of human population. One of the common
bacterial infection is Urinary tract infection (UTI), also called as bladder infection or acute cystitis. The causal agents include
Escherichia coli, Klebsiella, Proteus, Pseudomonas, Staphylococcus, Saprophyticus and Enterobacter. The drug resistant bacterial
strains are currently a major health concern in treating bacterial infections. The β-lactam antibiotics are generally prescribed to treat
UTI infections. Due to their extensive and persistent usage, it has led to worldwide appearance of drug-resistant strains. Bacteria
have developed resistance to β-lactams by two mainmechanisms: the production of β-lactamases (βL), sometimes accompanied by
a decrease of outer membrane permeability, and the production of low-affinity, drug resistant Penicillin Binding Proteins (PBPs).
The major challenge for the new drug is to be unsusceptible to the action of βL. Medicinal plants reveal important pharmacological
activities for developing novel therapeutic antibacterial agents. Fifteen phytochemicals were selected for the molecular docking
simulation based on their antibacterial activity. The βL in this study (TEM, OXA and AmpC) have been selected as targets. The
interactions between ligands and the selected proteins were observed for different poses. The potent compound having the best
docking score and good interactions with the protein has been studied. The molecular simulation data were further confirmed by
nitrocefin assay to prove the inhibitory potential towards βL. Among all phytochemicals, chlorogenic acid was found to be the most
potent βL inhibitor.